Scientific Reports Biology Table of Contents e-alert: August 2013

01 August 2013      • Visit Scientific Reports • Submit your manuscript to Scientific Reports     Welcome to your Table of Contents e-alert for Scientific Reports               Biological Sciences               High-Resolution Temporal Response Patterns to Influenza Vaccine Reveal a Distinct Human Plasma Cell Gene Signature ▶     Alicia D. Henn, Shuang Wu, Xing Qiu et al.     To identify sources of inter-subject variation in vaccine responses, we performed high-frequency sampling of human peripheral blood cells post-vaccination, followed by a novel …             Studying Cancer Stem Cell Dynamics on PDMS Surfaces for Microfluidics Device Design ▶     Weijia Zhang, Dong Soon Choi, Yen H. Nguyen et al.     This systematic study clarified a few interfacial aspects of cancer cell phenotypes on polydimethylsiloxane (PDMS) substrates and indicated that the cell phenotypic equilibrium …             Identification of highly-disrupted tRNA genes in nuclear genome of the red alga, Cyanidioschyzon merolae 10D ▶     Akiko Soma, Junichi Sugahara, Akinori Onodera et al.     The limited locations of tRNA introns are crucial for eukaryal tRNA-splicing endonuclease recognition. However, our analysis of the nuclear genome of an early-diverged red alga, …             Force produced after stretch in sarcomeres and half-sarcomeres isolated from skeletal muscles ▶     Fábio C. Minozzo, Bruno M. Baroni, José A. Correa et al.     The goal of this study was to evaluate if isolated sarcomeres and half-sarcomeres produce a long-lasting increase in force after a stretch is imposed during activation. Single and …             Affinity flow fractionation of cells via transient interactions with asymmetric molecular patterns ▶     Suman Bose, Rishi Singh, Mikhail Hanewich-Hollatz et al.     Flow fractionation of cells using physical fields to achieve lateral displacement finds wide applications, but its extension to surface molecule-specific separation requires …             Nuclear MTA1 overexpression is associated with aggressive prostate cancer, recurrence and metastasis in African Americans ▶     Steven J. Dias, Xinchun Zhou, Marina Ivanovic et al.     Metastasis-associated protein 1 (MTA1), a negative epigenetic modifier, plays a critical role in prostate cancer (PCa) progression. We hypothesized that MTA1 overexpression in …             Male soldiers are functional in the Japanese strain of a polyembryonic wasp ▶     Daisuke Uka, Azusa Takahashi-Nakaguchi, Jin Yoshimura et al.     Polyembryonic parasitoids clonally produce sterile soldier larvae in both sexes. Female soldier larvae of Copidosoma floridanum defend their siblings and host resources against …             Towards a better understanding of the novel avian-origin H7N9 influenza A virus in China ▶     Yongbo Wang, Zhangyan Dai, Han Cheng et al.     Recently, a highly dangerous bird flu has infected over 130 patients in China, and the outbreak was attributed to a novel avian-origin H7N9 virus. Here, we performed a systematic …             A disulphide bond in the E2 enzyme Pex4p modulates ubiquitin-conjugating activity ▶     Chris Williams, Marlene van den Berg, Will A. Stanley et al.     The ubiquitin-conjugating enzyme Pex4p together with its binding partner, the peroxisomal membrane protein Pex22p, co-ordinates cysteine-dependent ubiquitination of the cycling …             CO2-based in-line phase contrast imaging of small intestine in mice ▶     Rongbiao Tang, Wei-Xia Li, Wei Huang et al.     The objective of this study was to explore the potential of CO2 single contrast in-line phase contrast imaging (PCI) for pre-clinical small intestine investigation. The absorption …             Thymic Stromal Lymphopoietin Induces Migration in Human Airway Smooth Muscle Cells ▶     Naresh Singh Redhu, Lianyu Shan, Hesam Movassagh et al.     Airway remodeling due to increased airway smooth muscle (ASM) mass, likely due to enhanced migration and proliferation, has been shown to be highly associated with decline in lung …             Stochastic effects as a force to increase the complexity of signaling networks ▶     Hiroyuki Kuwahara, Xin Gao     Cellular signaling networks are complex and appear to include many nonfunctional elements. Recently, it was suggested that nonfunctional interactions of proteins cause signaling …             Removing PCR for the elimination of undesired DNA fragments cycle by cycle ▶     Jiaojiao Huan, Kangkang Wan, Yunjun Liu et al.     A novel removing polymerase chain reaction (R-PCR) technique was developed, which can eliminate undesired genes, cycle by cycle, with efficiencies of 60.9% (cDNAs), 73.6% (genomic …             Binding of disordered proteins to a protein hub ▶     Elio A. Cino, Ryan C. Killoran, Mikko Karttunen et al.     A small number of proteins, called hubs, have high connectivity and are essential for interactome functionality and integrity. Keap1 is a crucial hub in the oxidative stress …             Biological soliton in multicellular movement ▶     Hidekazu Kuwayama, Shuji Ishida     Solitons have been observed in various physical phenomena. Here, we show that the distinct characteristics of solitons are present in the mass cell movement of non-chemotactic …             Mining large-scale response networks reveals 'topmost activities' in Mycobacterium tuberculosis infection ▶     Awanti Sambarey, Karyala Prashanthi, Nagasuma Chandra     Mycobacterium tuberculosis owes its high pathogenic potential to its ability to evade host immune responses and thrive inside the macrophage. The outcome of infection is largely …             Prefrontal cortical microcircuits bind perception to executive control ▶     Ioan Opris, Lucas Santos, Greg A. Gerhardt et al.     During the perception-to-action cycle, our cerebral cortex mediates the interactions between the environment and the perceptual-executive systems of the brain. At the top of the …             Size-controlled conformal nanofabrication of biotemplated three-dimensional TiO2 and ZnO nanonetworks ▶     Hakan Ceylan, Cagla Ozgit-Akgun, Turan S. Erkal et al.     A solvent-free fabrication of TiO2 and ZnO nanonetworks is demonstrated by using supramolecular nanotemplates with high coating conformity, uniformity, and atomic scale size …             Rapid on-site/in-situ detection of heavy metal ions in environmental water using a structure-switching DNA optical biosensor ▶     Feng Long, Anna Zhu, Hanchang Shi et al.     A structure-switching DNA optical biosensor for rapid on-site/in situ detection of heavy metal ions is reported. Mercury ions (Hg2), highly toxic and ubiquitous pollutants, were …             Development associated profiling of chitinase and microRNA of Helicoverpa armigera identified chitinase repressive microRNA ▶     Neema Agrawal, Bindiya Sachdev, Janneth Rodrigues et al.     Expression of chitinase is developmentally regulated in insects in consonance with their molting process. During the larval-larval metamorphosis in Helicoverpa armigera, chitinase …             Mass fabrication and delivery of 3D multilayer μTags into living cells ▶     Lisa Y. Chen, Kokab B. Parizi, Hisanori Kosuge et al.     Continuous monitoring of in vivo biological processes and their evolution at the cellular level would enable major advances in our understanding of biology and disease. As a …             Whole-cell phase contrast imaging at the nanoscale using Fresnel Coherent Diffractive Imaging Tomography ▶     Michael W. M. Jones, Grant A. van Riessen, Brian Abbey et al.     X-ray tomography can provide structural information of whole cells in close to their native state. Radiation-induced damage, however, imposes a practical limit to image …             Signaling efficiency of Gαq through its effectors p63RhoGEF and GEFT depends on their subcellular location ▶     Joachim Goedhart, Jakobus van Unen, Merel J. W. Adjobo-Hermans et al.     The p63RhoGEF and GEFT proteins are encoded by the same gene and both members of the Dbl family of guanine nucleotide exchange factors. These proteins can be activated by the …             Predator-induced changes in the growth of eyes and false eyespots ▶     Oona M. Lönnstedt, Mark I. McCormick, Douglas P. Chivers     The animal world is full of brilliant colours and striking patterns that serve to hide individuals or attract the attention of others. False eyespots are pervasive across a …             Ferroelectric Polarization in Nanocrystalline Hydroxyapatite Thin Films on Silicon ▶     S. B. Lang, S. A. M. Tofail, A. L. Kholkin et al.     Hydroxyapatite nanocrystals in natural form are a major component of bone- a known piezoelectric material. Synthetic hydroxyapatite is widely used in bone grafts and prosthetic …             Novel Insights into Breast Cancer Genetic Variance through RNA Sequencing ▶     Anelia Horvath, Suresh Babu Pakala, Prakriti Mudvari et al.     Using RNA sequencing of triple-negative breast cancer (TNBC), non-TBNC and HER2-positive breast cancer sub-types, here we report novel expressed variants, allelic prevalence and …             Single blastomere expression profiling of Xenopus laevis embryos of 8 to 32-cells reveals developmental asymmetry ▶     Monika Flachsova, Radek Sindelka, Mikael Kubista     We have measured the expression of 41 maternal mRNAs in individual blastomeres collected from the 8 to 32-cell Xenopus laevis embryos to determine when and how asymmetry in the …             Improved identification of wheat gluten proteins through alkylation of cysteine residues and peptide-based mass spectrometry ▶     Ine Rombouts, Bert Lagrain, Markus Brunnbauer et al.     The concentration and composition of wheat gluten proteins and the presence, concentration and location of cysteine residues therein are important for wheat flour quality. …             Sprouty genes regulate proliferation and survival of human embryonic stem cells ▶     Hady Felfly, Ophir D. Klein     Sprouty (Spry) genes encode negative regulators of receptor tyrosine kinase (RTK) signaling, which plays important roles in human embryonic stem cells (hESCs). SPRY2 and SPRY4 are …             Spatio-temporal analysis of type 2 diabetes mellitus based on differential expression networks ▶     Shao-Yan Sun, Zhi-Ping Liu, Tao Zeng et al.     T2DM is complex in its dynamical dependence on multiple tissues, disease states, and factors' interactions. However, most existing work devoted to characterizing its …             A patterned recombinant human IgM guides neurite outgrowth of CNS neurons ▶     Xiaohua Xu, Nathan J. Wittenberg, Luke R. Jordan et al.     Matrix molecules convey biochemical and physical guiding signals to neurons in the central nervous system (CNS) and shape the trajectory of neuronal fibers that constitute neural …             Polyreactive Antibodies Plus Complement Enhance the Phagocytosis of Cells Made Apoptotic by UV-Light or HIV ▶     Zhao-hua Zhou, Teresa Wild, Ying Xiong et al.     Polyreactive antibodies are a major component of the natural antibody repertoire and are capable of binding a variety of structurally unrelated antigens. Many of the properties …             Fast and accurate automated cell boundary determination for fluorescence microscopy ▶     Stephen Hugo Arce, Pei-Hsun Wu, Yiider Tseng     Detailed measurement of cell phenotype information from digital fluorescence images has the potential to greatly advance biomedicine in various disciplines such as patient …             Competitive Binding of Natural Amphiphiles with Graphene Derivatives ▶     Slaven Radic, Nicholas K. Geitner, Ramakrishna Podila et al.     Understanding the transformation of graphene derivatives by natural amphiphiles is essential for elucidating the biological and environmental implications of this emerging class …             Regulation of homocysteine metabolism by Mycobacterium tuberculosis S-adenosylhomocysteine hydrolase ▶     Anshika Singhal, Gunjan Arora, Andaleeb Sajid et al.     Mycobacterium tuberculosis modulates expression of various metabolism-related genes to adapt in the adverse host environment. The gene coding for M. tuberculosis …             GSTM1 and GSTT1 null polymorphisms and male infertility risk: an updated meta-analysis encompassing 6934 subjects ▶     Wei Wu, Jing Lu, Qiuqin Tang et al.     Published data on the association between the GST genes polymorphisms and male infertility risk are inconclusive. We investigated GST genes polymorphisms in a large sample size …             Virus infection of a weed increases vector attraction to and vector fitness on the weed ▶     Gong Chen, Huipeng Pan, Wen Xie et al.     Weeds are important in the ecology of field crops, and when crops are harvested, weeds often become the main hosts for plant viruses and their insect vectors. Few studies, …             Molecular logistics using cytocleavable polyrotaxanes for the reactivation of enzymes delivered in living cells ▶     Atsushi Tamura, Go Ikeda, Ji-Hun Seo et al.     The intracellular delivery of enzymes is an essential methodology to extend their therapeutic application. Herein, we have developed dissociable supermolecule-enzyme …             Ultrasound-enhanced delivery of Morpholino with Bubble liposomes ameliorates the myotonia of myotonic dystrophy model mice ▶     Michinori Koebis, Tamami Kiyatake, Hiroshi Yamaura et al.     Phosphorodiamidate morpholino oligonucleotide (PMO)-mediated control of the alternative splicing of the chloride channel 1 (CLCN1) gene is a promising treatment for myotonic …             A simple method for deriving functional MSCs and applied for osteogenesis in 3D scaffolds ▶     Lijin Zou, Yonglun Luo, Muwan Chen et al.     We describe a simple method for bone engineering using biodegradable scaffolds with mesenchymal stem cells derived from human induced-pluripotent stem cells (hiPS-MSCs). The …             High arsenic in rice is associated with elevated genotoxic effects in humans ▶     Mayukh Banerjee, Nilanjana Banerjee, Pritha Bhattacharjee et al.     Arsenic in drinking water may cause major deleterious health impacts including death. Although arsenic in rice has recently been demonstrated to be a potential exposure route for …             The impact of p53 in predicting clinical outcome of breast cancer patients with visceral metastasis ▶     P. Yang, C. W. Du, M. Kwan et al.     In the study, we analyzed role of p53 in predicting outcome in visceral metastasis breast cancer (VMBC) patients. 97 consecutive VMBC patients were studied. P53 positivity rate …             Acinetobacter phage genome is similar to Sphinx 2.36, the circular DNA copurified with TSE infected particles ▶     Toshisangba Longkumer, Swetha Kamireddy, Venkateswar Reddy Muthyala et al.     While analyzing plasmids of Acinetobacter sp. DS002 we have detected a circular DNA molecule pTS236, which upon further investigation is identified as the genome of a phage. The …             Two-photon voltage imaging using a genetically encoded voltage indicator ▶     Walther Akemann, Mari Sasaki, Hiroki Mutoh et al.     Voltage-sensitive fluorescent proteins (VSFPs) are a family of genetically-encoded voltage indicators (GEVIs) reporting membrane voltage fluctuation from genetically-targeted …             Enhancing gold recovery from electronic waste via lixiviant metabolic engineering in Chromobacterium violaceum ▶     Song Buck Tay, Gayathri Natarajan, Muhammad Nadjad bin Abdul Rahim et al.     Conventional leaching (extraction) methods for gold recovery from electronic waste involve the use of strong acids and pose considerable threat to the environment. The alternative …             H2O2-responsive molecularly engineered polymer nanoparticles as ischemia/reperfusion-targeted nanotherapeutic agents ▶     Dongwon Lee, Soochan Bae, Donghyun Hong et al.     The main culprit in the pathogenesis of ischemia/reperfusion (I/R) injury is the overproduction of reactive oxygen species (ROS). Hydrogen peroxide (H2O2), the most abundant form …             Nanosensor dosimetry of mouse blood proteins after exposure to ionizing radiation ▶     Dokyoon Kim, Francesco Marchetti, Zuxiong Chen et al.     Giant magnetoresistive (GMR) nanosensors provide a novel approach for measuring protein concentrations in blood for medical diagnosis. Using an in vivo mouse radiation model, we …             Citizen Science: linking the recent rapid advances of plant flowering in Canada with climate variability ▶     Alemu Gonsamo, Jing M. Chen, Chaoyang Wu     The timing of crucial events in plant life cycles is shifting in response to climate change. We use phenology records from PlantWatch Canada 'Citizen Science' networks to study …             Differential effects of an exotic plant virus on its two closely related vectors ▶     Huipeng Pan, Dong Chu, Baiming Liu et al.     Concurrent spread of Tomato yellow leaf curl virus (TYLCV) with invasion of Bemisia tabaci Q rather than B in China suggests a more mutualistic relationship between TYLCV and Q …             Cortical stimulation consolidates and reactivates visual experience: neural plasticity from magnetic entrainment of visual activity ▶     Hsin-I Liao, Daw-An Wu, Neil Halelamien et al.     Delivering transcranial magnetic stimulation (TMS) shortly after the end of a visual stimulus can cause a TMS-induced 'replay' or 'visual echo' of the visual percept. In the …             Memory suppression trades prolonged fear and sleep-dependent fear plasticity for the avoidance of current fear ▶     Kenichi Kuriyama, Motoyasu Honma, Takuya Yoshiike et al.     Sleep deprivation immediately following an aversive event reduces fear by preventing memory consolidation during homeostatic sleep. This suggests that acute insomnia might act …             Noninvasive fractal biomarker of clock neurotransmitter disturbance in humans with dementia ▶     Kun Hu, David G. Harper, Steven A. Shea et al.     Human motor activity has a robust, intrinsic fractal structure with similar patterns from minutes to hours. The fractal activity patterns appear to be physiologically important …             Discovery of a kernel for controlling biomolecular regulatory networks ▶     Junil Kim, Sang-Min Park, Kwang-Hyun Cho     Cellular behavior is determined not by a single molecule but by many molecules that interact strongly with one another and form a complex network. It is unclear whether cellular …             Fluoxetine treatment promotes functional recovery in a rat model of cervical spinal cord injury ▶     Manuela Scali, Tatjana Begenisic, Marco Mainardi et al.     Spinal cord injury (SCI) is a severe condition leading to enduring motor deficits. When lesions are incomplete, promoting spinal cord plasticity might be a useful strategy to …             Local dynamics in decision making: The evolution of preference within and across decisions ▶     Denis O'Hora, Rick Dale, Petri T. Piiroinen et al.     Within decisions, perceived alternatives compete until one is preferred. Across decisions, the playing field on which these alternatives compete evolves to favor certain …             Exploring the limits of community detection strategies in complex networks ▶     Rodrigo Aldecoa, Ignacio Marín     The characterization of network community structure has profound implications in several scientific areas. Therefore, testing the algorithms developed to establish the optimal …             Human mobility and the worldwide impact of intentional localized highly pathogenic virus release ▶     Bruno Gonçalves, Duygu Balcan, Alessandro Vespignani     The threat of bioterrorism and the possibility of accidental release have spawned a growth of interest in modeling the course of the release of a highly pathogenic agent. Studies …             Neutral buoyancy is optimal to minimize the cost of transport in horizontally swimming seals ▶     Katsufumi Sato, Kagari Aoki, Yuuki Y. Watanabe et al.     Flying and terrestrial animals should spend energy to move while supporting their weight against gravity. On the other hand, supported by buoyancy, aquatic animals can minimize …             Zn- and Mg- Containing Tricalcium Phosphates-Based Adjuvants for Cancer Immunotherapy ▶     Xiupeng Wang, Xia Li, Kazuo Onuma et al.     Zn-, and Mg-containing tricalcium phosphates (TCPs) loaded with a hydrothermal extract of a human tubercle bacillus (HTB) were prepared by immersing Zn-TCP and Mg-TCP in …             S-nitrosylation regulates mitochondrial quality control via activation of parkin ▶     Kentaro Ozawa, Akira T. Komatsubara, Yuhei Nishimura et al.     Parkin, a ubiquitin E3 ligase of the ring between ring fingers family, has been implicated in mitochondrial quality control. A series of recent reports have suggested that the …             DNA replication-dependent binding of CTCF plays a critical role in adenovirus genome functions ▶     Tetsuro Komatsu, Takeshi Sekiya, Kyosuke Nagata     The expression of adenovirus late genes is shown to require viral DNA replication, but its mechanism remains elusive. Here we found that knockdown of CTCF suppresses viral DNA …             Infection with retroviral vectors leads to perturbed DNA replication increasing vector integrations into fragile sites ▶     Assaf C. Bester, Moshe Kafri, Karin Maoz et al.     Genome instability is a hallmark of cancer. Common fragile sites (CFSs) are specific regions in the human genome that are sensitive to replication stress and are prone to genomic …             Spt6 prevents transcription-coupled loss of posttranslationally modified histone H3 ▶     Hiroaki Kato, Kosuke Okazaki, Tetsushi Iida et al.     The tail of histone H3 is an ideal medium for storing epigenetic information because displacement of histone H3 is heavily restricted during transcription. To maintain the …             Improved network community structure improves function prediction ▶     Juyong Lee, Steven P. Gross, Jooyoung Lee     We are overwhelmed by experimental data, and need better ways to understand large interaction datasets. While clustering related nodes in such networks—known as community …             Initial Pulmonary Respiration Causes Massive Diaphragm Damage and Hyper-CKemia in Duchenne Muscular Dystrophy Dog ▶     Akinori Nakamura, Masanori Kobayashi, Mutsuki Kuraoka et al.     The molecular mechanism of muscle degeneration in a lethal muscle disorder Duchene muscular dystrophy (DMD) has not been fully elucidated. The dystrophic dog, a model of DMD, …             Mimicking cellular transport mechanism in stem cells through endosomal escape of new peptide-coated quantum dots ▶     Karthikeyan Narayanan, Swee Kuan Yen, Qingqing Dou et al.     Protein transport is an important phenomenon in biological systems. Proteins are transported via several mechanisms to reach their destined compartment of cell for its complete …             Extreme alien light allows survival of terrestrial bacteria ▶     Neil Johnson, Guannan Zhao, Felipe Caycedo et al.     Photosynthetic organisms provide a crucial coupling between the Sun's energy and metabolic processes supporting life on Earth. Searches for extraterrestrial life focus on seeking …             Natural channel protein inserts and functions in a completely artificial, solid-supported bilayer membrane ▶     Xiaoyan Zhang, Wangyang Fu, Cornelia G. Palivan et al.     Reconstitution of membrane proteins in artificial membrane systems creates a platform for exploring their potential for pharmacological or biotechnological applications. …             Modulation of Post-Antibiotic Bacterial Community Reassembly and Host Response by Candida albicans ▶     John R. Erb Downward, Nicole R. Falkowski, Katie L. Mason et al.     The introduction of Candida albicans into cefoperazone-treated mice results in changes in bacterial community reassembly. Our objective was to use high-throughput sequencing to …             In Situ Breast Cancer and Microchimerism ▶     Jinny K. Eun, Katherine A. Guthrie, Gary Zirpoli et al.     Microchimeric cells of fetal origin persistent in the maternal circulation post-partum are associated with protection against invasive breast cancer. Here using quantitative …             Modeling highly pathogenic avian influenza transmission in wild birds and poultry in West Bengal, India ▶     Pranav S. Pandit, David A. Bunn, Satish A. Pande et al.     Wild birds are suspected to have played a role in highly pathogenic avian influenza (HPAI) H5N1 outbreaks in West Bengal. Cluster analysis showed that H5N1 was introduced in West …             Acoustic tweezing cytometry for live-cell subcellular modulation of intracellular cytoskeleton contractility ▶     Zhenzhen Fan, Yubing Sun, Di Chen et al.     Mechanical forces are critical to modulate cell spreading, contractility, gene expression, and even stem cell differentiation. Yet, existing tools that can apply controllable …             Collagen morphology and texture analysis: from statistics to classification ▶     Leila B. Mostaço-Guidolin, Alex C.-T. Ko, Fei Wang et al.     In this study we present an image analysis methodology capable of quantifying morphological changes in tissue collagen fibril organization caused by pathological conditions. …             Specific Accumulation of Lipid Droplets in Hepatocyte Nuclei of PFOA-exposed BALB/c Mice ▶     Ling Wang, Yu Wang, Yong Liang et al.     Lipid droplets (LDs), which are important storage structures for neutral lipids and organelles of diverse functions, participate in various cellular activities. In this study, …             Continuous DOPA synthesis from a single AAV: dosing and efficacy in models of Parkinson's disease ▶     Erik Cederfjäll, Nathalie Nilsson, Gurdal Sahin et al.     We used a single adeno-associated viral (AAV) vector co-expressing tyrosine hydroxylase (TH) and GTP cyclohydrolase 1 (GCH1) to investigate the relationship between vector dose, …             A practical method to detect SNVs and indels from whole genome and exome sequencing data ▶     Daichi Shigemizu, Akihiro Fujimoto, Shintaro Akiyama et al.     The recent development of massively parallel sequencing technology has allowed the creation of comprehensive catalogs of genetic variation. However, due to the relatively high …             Identification of Two Maternal Transmission Ratio Distortion Loci in Pedigrees of the Framingham Heart Study ▶     Yang Liu, Liangliang Zhang, Shuhua Xu et al.     Transmission ratio distortion (TRD) is indicated by the recovery of alleles in offspring in non-Mendelian proportions. An assumption of Mendelian proportion is central to many …             Generation of a functional liver tissue mimic using adipose stromal vascular fraction cell-derived vasculatures ▶     S. S. Nunes, J. G. Maijub, L. Krishnan et al.     One of the major challenges in cell implantation therapies is to promote integration of the microcirculation between the implanted cells and the host. We used adipose-derived …             Microsecond dynamics of an unfolded protein by a line confocal tracking of single molecule fluorescence ▶     Hiroyuki Oikawa, Yuta Suzuki, Masataka Saito et al.     We present a new method for high speed tracking of fluorescence time series from single proteins. The method uses a fast sample flow and a modified confocal microscopy, line …             Manumycin A corrects aberrant splicing of Clcn1 in myotonic dystrophy type 1 (DM1) mice ▶     Kosuke Oana, Yoko Oma, Satoshi Suo et al.     Myotonic dystrophy type 1 (DM1) is the most common muscular dystrophy in adults and as yet no cure for DM1. Here, we report the potential of manumycin A for a novel DM1 …             High levels of reactive oxygen species in gold nanoparticle-targeted cancer cells following femtosecond pulse irradiation ▶     Limor Minai, Daniella Yeheskely-Hayon, Dvir Yelin     Cancer cells could be locally damaged using specifically targeted gold nanoparticles and laser pulse irradiation, while maintaining minimum damage to nearby, particle-free tissue. …             Pannexin 1 involvement in bladder dysfunction in a multiple sclerosis model ▶     Hiromitsu Negoro, Sarah E. Lutz, Louis S. Liou et al.     Bladder dysfunction is common in Multiple Sclerosis (MS) but little is known of its pathophysiology. We show that mice with experimental autoimmune encephalomyelitis (EAE), a MS …             Optically Induced Thermal Gradients for Protein Characterization in Nanolitre-scale Samples in Microfluidic Devices ▶     D. M. Sagar, Samir Aoudjane, Matthieu Gaudet et al.     Proteins are the most vital biological functional units in every living cell. Measurement of protein stability is central to understanding their structure, function and role in …             High-speed atomic force microscopy combined with inverted optical microscopy for studying cellular events ▶     Yuki Suzuki, Nobuaki Sakai, Aiko Yoshida et al.     A hybrid atomic force microscopy (AFM)-optical fluorescence microscopy is a powerful tool for investigating cellular morphologies and events. However, the slow data acquisition …             Nuclear phosphatidylinositol-5-phosphate regulates ING2 stability at discrete chromatin targets in response to DNA damage ▶     Dennis J. Bua, Gloria Mas Martin, Olivier Binda et al.     ING2 (inhibitor of growth family member 2) is a component of a chromatin-regulatory complex that represses gene expression and is implicated in cellular processes that promote …             Ionic strength-dependent conformations of a ubiquitin-like small archaeal modifier protein (SAMP2) from Haloferax volcanii ▶     Shanhui Liao, Wen Zhang, Kai Fan et al.     Ubiquitin-like proteins play important roles in diverse biological processes. In this study, we present an unexpected finding that a ubiquitin-like small archaeal modifier protein …             Spiderweb deformation induced by electrostatically charged insects ▶     Victor Manuel Ortega-Jimenez, Robert Dudley     Capture success of spider webs has been associated with their microstructure, ornamentation, and wind-induced vibrations. Indirect evidence suggests that statically charged …             Vasoconstriction by Electrical Stimulation: New Approach to Control of Non-Compressible Hemorrhage ▶     Yossi Mandel, Richard Manivanh, Roopa Dalal et al.     Non-compressible hemorrhage is the most common preventable cause of death on battlefield and in civilian traumatic injuries. We report the use of microsecond pulses of electric …             High performance, LED powered, waveguide based total internal reflection microscopy ▶     Srinivasan Ramachandran, Daniel A. Cohen, Arjan P. Quist et al.     Total internal reflection fluorescence (TIRF) microscopy is a rapidly expanding optical technique with excellent surface sensitivity and limited background fluorescence. …             Rapid and accurate identification of in vivo-induced haploid seeds based on oil content in maize ▶     Albrecht E. Melchinger, Wolfgang Schipprack, Tobias Würschum et al.     The needs of a growing human population require rapid and efficient development of improved cultivars by plant breeders. The doubled haploid (DH) technology enables generating …             The role of ear stone size in hair cell acoustic sensory transduction ▶     Maya Inoue, Masashi Tanimoto, Yoichi Oda     Hearing and bodily balance are different sensations initiated by a common mechanism. Both sound- and head movement-dependent mechanical displacement are converted into electrical …             DNA Methylation Regulated Nucleosome Dynamics ▶     Isabel Jimenez-Useche, Jiaying Ke, Yuqing Tian et al.     A strong correlation between nucleosome positioning and DNA methylation patterns has been reported in literature. However, the mechanistic model accounting for the correlation …             Small molecule drug screening in Drosophila identifies the 5HT2A receptor as a feeding modulation target ▶     Gabriel Gasque, Stephen Conway, Juan Huang et al.     Dysregulation of eating behavior can lead to obesity, which affects 10% of the adult population worldwide and accounts for nearly 3 million deaths every year. Despite this burden …             Location- and sex-specific differences in weight and motor coordination in two commonly used mouse strains ▶     Attila D. Kovács, David A. Pearce     Several studies have shown that environmental factors can affect the outcome of behavioral experiments, shedding doubts on the inter-laboratory reproducibility of behavioral test …             Quantitative analysis of molecular-level DNA crystal growth on a 2D surface ▶     Junwye Lee, Shogo Hamada, Si Un Hwang et al.     Crystallization is an essential process for understanding a molecule's aggregation behavior. It provides basic information on crystals, including their nucleation and growth …             Role of Huge Geometric Circular Structures in the Reproduction of a Marine Pufferfish ▶     Hiroshi Kawase, Yoji Okata, Kimiaki Ito     We report that male pufferfishes (Torquigener sp., Tetraodontidae) constructed large geometric circular structures on the seabed that played an important role in female mate …             Extraordinary expansion of a Sorangium cellulosum genome from an alkaline milieu ▶     Kui Han, Zhi-feng Li, Ran Peng et al.     Complex environmental conditions can significantly affect bacterial genome size by unknown mechanisms. The So0157-2 strain of Sorangium cellulosum is an alkaline-adaptive …             In Vivo and Real-time Monitoring of Secondary Metabolites of Living Organisms by Mass Spectrometry ▶     Bin Hu, Lei Wang, Wen-Cai Ye et al.     Secondary metabolites are compounds that are important for the survival and propagation of animals and plants. Our current understanding on the roles and secretion mechanism of …             Synaptic transmission and the susceptibility of HIV infection to anti-viral drugs ▶     Natalia L. Komarova, David N. Levy, Dominik Wodarz     Cell-to-cell viral transmission via virological synapses has been argued to reduce susceptibility of the virus population to anti-viral drugs through multiple infection of cells, …             MICE Models: Superior to the HERG Model in Predicting Torsade de Pointes ▶     James Kramer, Carlos A. Obejero-Paz, Glenn Myatt et al.     Drug-induced block of the cardiac hERG (human Ether-à-go-go-Related Gene) potassium channel delays cardiac repolarization and increases the risk of Torsade de Pointes (TdP), a …                 Nature Immunology  FOCUS ON MICROBIOTA   This series of specially commissioned articles discuss the reciprocal regulation between the host immune system and commensal microbiota, the dynamic interactions between commensals and pathogens, and emerging information on how resident viruses might influence immune homeostasis.          • Careers & Jobs           naturejobs.com Science jobs of the week       Research Technician     EMBL (European Molecular Biology Laboratory)            Postdoctoral Researcher     The Ohio State University College of Medicine            Research Associate     UCL            Junior Research / Travel Fellowships     The Kay Kendall Leukaemia Fund            Research Associate, Basic Sciences     Fred Hutchinson Cancer Research Center            Research Asst I     MD Anderson Cancer Center            Post Doctoral Research Fellow     University of Canterbury, New Zealand            Junior Research Group     University of Tübingen, Faculty of Medicine            Clinical Trials Associate     Lunenfeld - Tanenbaum Research Institute            Researcher and Instructor     Koc University School of Medicine          No matter what your career stage, student, postdoc or senior scientist, you will find articles on naturejobs.com to help guide you in your science career. 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