Nature Methods Application Notes e-UPDATE: 10 September 2013

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10 SEPTEMBER 2013  Application Notes bring you the latest information about innovative tools and technologies and their applications in the lab. We hope that you will find this service useful and informative and encourage you to sign up for future Updates to ensure that you never miss one!

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FEATURED APPLICATION NOTE Faster, cleaner bisulfite conversion that yields more intact DNA for downstream analysis www.promega.com > Performing DNA purification, bisulfite conversion and amplification are crucial steps in DNA methylation analysis, especially when working with difficult sample types like formalin-fixed paraffin-embedded (FFPE) tissue. The MethylEdge™ Bisulfite Conversion System provides a rapid, efficient method for bisulfite conversion with minimal DNA fragmentation in less than 2 h. Coupled with purification chemistries optimized for FFPE tissue and flexible, robust amplification technologies for detection, the MethylEdge™ Bisulfite Conversion System delivers high-quality converted DNA for downstream analysis. PDF

		Endotoxin-free protein production—ClearColi™ technology www.lucigen.com > A new Escherichia coli strain with a genetically modified lipopolysaccharide (LPS) molecule has been created that enables protein expression without the endotoxin. Proteins expressed and purified from ClearColi™ BL21(DE3) cells will not trigger LPS-related immune response in human cells, making them ideal for immunogenicity, toxicity and cytokine assays. PDF

		A transposable approach to RNA-seq from total RNA www.epibio.com > The rapid progress of RNA-seq technology requires the development of simple, versatile tools that competently reflect RNA levels in the cell. We have developed the TotalScript™ RNA-Seq Kit, a new, transposome-based directional library prep method suitable for a variety of RNA sample types and a range of input. TotalScript libraries exhibit high complexity, ~97–99% directionality and low rRNA contamination. The method is scalable and robust, allowing input amounts of ~100 pg of poly(A)+ RNA or ~5 ng of total RNA. PDF

		Mosquito® www.ttplabtech.com > A simple and automated solution for miniaturising reaction volumes for Nextera NGS sample preparation. PDF

		Peggy™: size- or charge-based western blotting at the push of a button www.proteinsimple.com > http://www.sbhdiagnostics.com > Western blotting continues to be the most popular method used for protein analysis, in spite of its drawbacks in quantitation and reproducibility and its laborious workflow. Protein Simple's platform of Simple Western™ products provides a new standard in instrumentation that fully automates the archaic western blotting process and provides highly reproducible and quantitative data. Now Peggy™, the newest addition to the Simple Western™ family, provides immunoassays with separation by size or charge in a single instrument. PDF

		RNAzol® BD: a reagent for the effective isolation of RNA from whole blood www.mrcgene.com > Previously, we introduced an advanced version of the single-step method for RNA extraction. We have now adapted this methodology for the isolation of total RNA, mRNA, small RNA and microRNA from whole blood, plasma or serum of human and animal origin. This new reagent, RNAzol® BD, provides the highest yield and purity of blood-derived RNA, and it also allows for the simultaneous isolation of DNA from samples used for RNA isolation. PDF

		Improved RNA-seq of blood-derived RNA increases gene discovery and coverage www.epibio.com > RNA-seq of blood-derived RNA can aid discovery of the cause of disease, as well as preclinical research. However, the high content of globin mRNA and ribosomal RNA (rRNA) in blood samples limits the detection of rare transcripts and splice variants. The ScriptSeq™ Complete Kit (Blood) is a new method to prepare directional RNA-seq libraries that are virtually free of globin mRNA and rRNA contamination and exhibit high detection of reference and novel genes. PDF

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